-
-
實驗服務
-
新聞資訊
Monoclonal Anti-GST-Peroxidase (HRP) Conjugated
PRODUCT NAME：Monoclonal Anti-GST-Peroxidase (HRP) Conjugated
CATALOG NUMBER：U33-890A-S
LOT NUMBER：U33022
ISOTYPE：Mouse IgG2b
Clone NO：1B20
SOURCE：Mouse Ascites
Purification：Protein A purified
QUANTITY：100μl
DESCRIPTION：Monoclonal antibodies are produced by immunizing mouse with GST protein.
APPLICATION：Western Blot
REAGENT：1×PBS with 50% glycerol
PROTEIN CONCENTRATION：approximately 1mg/ml
RECOMMENDED ANTIBODY DILUTION：Western Blot: 1:1000
STORAGE/STABILITY：Antibody-HRP is recommended being stored at -20℃.
Background:
Glutathione S-transferase (GST) was originally cloned from parasite Schistosoma japonicum and it is now a widely used protein fusion partner. Vectors containing GST Tag have been developed for both prokaryotic and eukaryotic systems. The GST fusion proteins are easily purified from cell lysates by affinity chromatography using Glutathione Sepharose 4B and eluting out the GST fusion protein from the column with denaturing form of glutathione. Using an anti-GST-peroxidase conjugated antibody provides a simple solution to detect the expression of GST fusion protein.