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Anti-β-Actin Monoclonal Antibody 

PRODUCT NAME：Anti-β-actin Monoclonal Antibody 

CATALOG NUMBER：U33-386A 

LOT NUMBER：U33022 

ISOTYPE：Mouse IgG1 

Clone NO：1B20 

SOURCE：Mouse Ascites  

QUANTITY：400ul

DESCRIPTION：Monoclonal antibody is produced by immunizing mouse with synthetic peptide [D(Ac)DDIAALVIDNGSGLC], coupled to KLH. 

REACTIVITY：human, mouse, African green monkey 

APPLICATIONS：Western Blot, Immunoprecipitation 

BUFFER：1×PBS with 50% glycerol 

PROTEIN CONCENTRATION：0.5 mg/ml 

APPLICATION NOTES：Western Blot: 0.5μg/ml 

STORAGE/STABILITY：Antibody is recommended being stored at -20℃. Avoid repeated freeze-thaw cycle.

 Background:

 Actin is one of the most conserved eukaryotic proteins. It is expressed in mammals and birds with at least six isoforms. There are three α-actins (skeletal, cardiac, and smooth muscle), one β-actin (β-nonmuscle), and two γ-actins (γ-smooth muscle and γ-nonmuscle). Our anti-β-actin monoclonal antibody provides a specific and useful tool in studying the intracellular distribution of β-actin and the static and dynamic aspects of the cytoskeleton.