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Anti-Flag agrose beads/Anti-Flag Affinity Gel

PRODUCT NAME：Anti-Flag agrose beads/Anti-Flag Affinity Gel

CATALOG NUMBER： U33-135A-F

LOT NUMBER：U33022

ISOTYPE：IgG2b 

Clone NO：1B20

QUANTITY：5ml

DESCRIPTION：Anti-Flag Affinity Gel is a purified murine IgG2b Monoclonal antibody covalently attached to sepharose 4B

APPLICATION：rProtein Purification, Immunoprecipitation

REAGENT：Supplied as a 50% suspension in 50% glycerol with 10mM sodium phosphate, 150mM sodium chloride, pH 7.4, containing 0.02% (w/v) sodium azide.

ANTIBODY BOUND：NLT 7.5 grams antibody/ Liter gel

RECOMMENDED VOLUME：IP: 5μl gel for 500μl crude protein solution

BINDING CAPACITY：Minimum 1.1mg of a Flag fusion protein eluted per ml of packed resin

SETTING VOLUME：10 ml resin slurry

STORAGE/STABILITY：

Anti-Flag Affinity Gel should be stored in 50% glycerol at -20℃ for maximum stability. The unopened product is stable for one year when stored as indicated. After use, the resin should be cleaned and stored in 50% glycerol with 10mM sodium phosphate, 150mM sodium chloride, pH 7.4, containing 0.02% sodium azide to protect the product. Do not freeze in the absence of glycerol