雜志名稱(chēng)：Frontiers in Immunology
影響因子：5.7
文章題目：Predicting acute lung injury in infants with congenital heart disease after cardiopulmonary bypass by gut microbiota
DOI：https://doi.org/10.3389/fimmu.2024.1362040
第一作者：Lan Jiang, Yueshuang Cun, Qiang Wang, Kede Wu, Menglong Hu, Zhen Wu, Tianyi Zhu, Zhaocong Yang, Nishant Patel, Xinyu Cai, Jirong Qi, Xuming Mo
作者單位：
南京醫(yī)科大學(xué)兒童醫(yī)院
南京醫(yī)科大學(xué)公共衛(wèi)生學(xué)院
引用YOBIBIO產(chǎn)品：
U96-1510E Human IL-6 ELISA Kit
U96-1513E Human IL-8 ELISA Kit
U96-6012E Human HMGB1 ELISA kit
文章摘要：
BackgroundAcute lung injury (ALI) is a serious and common complication that occurs in children with congenital heart disease after cardiopulmonary bypass (CPB) surgery, leading to higher mortality rates and poorer prognosis. Currently, there is no reliable predictive strategy for CPB-associated lung injury (CPB-ALI) in infants. Certain characteristics of the gut microbiota could potentially serve as biomarkers for predicting the development of CPB-ALI.MethodsWe conducted 16S rRNA sequencing to analyze the characteristics of the intestinal microbiota in healthy controls and infants with CHD admitted to the hospital. The CHD infants were divided into CPB-ALI and non-ALI (CPB-NALI) groups based on postoperative outcomes. Bacterial functional pathway prediction analysis was performed using PIRCUSt2, and the gut microbiota composition associated with immune status was determined with heatmap. Random forest regression models and ROC curves were utilized to predict the occurrence of CPB-ALI.ResultsOur study revealed significantly different microbiota compositions among three groups (CON, CPB-ALI, and CPB-NALI). The microbiota diversity was low in the CPB-ALI group with high pathogen abundance and significant decrease in Bacteroides, while the opposite was observed in the CPB-NALI group. The microbiota dysbiosis index was high in the CPB-ALI group, with its dominant microbiota significantly associated with multiple metabolic pathways. Additionally, CPB-ALI patients showed high levels of inflammatory cytokines IL-8 and HMGB1 in their serum, with high expression of IL-8 being associated with Enterobacteriaceae. Further correlation analysis showed that the differences in gut bacterial taxonomy were related to the occurrence of ALI, length of stay in the cardiac care unit, and ventilation time. It is noteworthy that Escherichia Shigella performed best in distinguishing CPB-ALI patients from non-ALI patients.ConclusionsOur study suggests that postoperative ALI patients have distinct gut microbiota upon admission compared to non-ALI patients after surgery. Dysbiosis of the gut microbiota may potentially impact the progression of ALI through metabolic pathways, quorum sensing, and the levels of inflammatory factors expressed in the serum. Escherichia Shigella represents a potential predictive factor for the occurrence of ALI in CHD infants after surgery. Acute lung injury, congenital heart disease, cardiopulmonary bypass surgery, gut microbiota, biomarker
背景急性肺損傷 （ALI） 是體外循環(huán) （CPB） 手術(shù)后先天性心臟病患兒發(fā)生的一種嚴(yán)重且常見(jiàn)的并發(fā)癥，導(dǎo)致死亡率較高，預(yù)后較差。目前，嬰兒 CPB 相關(guān)肺損傷 （CPB-ALI） 尚無(wú)可靠的預(yù)測(cè)策略。腸道微生物群的某些特征可能作為預(yù)測(cè) CPB-ALI 發(fā)展的生物標(biāo)志物。方法我們進(jìn)行 16S rRNA 測(cè)序，分析健康對(duì)照和住院 CHD 嬰兒腸道菌群特征。根據(jù)術(shù)后結(jié)局將 CHD 嬰兒分為 CPB-ALI 組和非 ALI （CPB-NALI） 組。使用 PIRCUSt2 進(jìn)行細(xì)菌功能通路預(yù)測(cè)分析，并用熱圖確定與免疫狀態(tài)相關(guān)的腸道菌群組成。采用隨機(jī)森林回歸模型和 ROC 曲線預(yù)測(cè) CPB-ALI 的發(fā)生。結(jié)果我們的研究揭示了三組 （CON 、 CPB-ALI 和 CPB-NALI） 之間的微生物群組成存在顯著差異。CPB-ALI 組微生物群多樣性低，病原體豐度高，擬桿菌屬顯著減少，而 CPB-NALI 組則相反。CPB-ALI 組菌群失調(diào)指數(shù)較高，其優(yōu)勢(shì)菌群與多種代謝途徑顯著相關(guān)。此外，CPB-ALI 患者的血清中炎性細(xì)胞因子 IL-8 和 HMGB1 水平較高，其中 IL-8 的高表達(dá)與腸桿菌科有關(guān)。 進(jìn)一步的相關(guān)性分析顯示，腸道細(xì)菌分類(lèi)學(xué)的差異與 ALI 的發(fā)生、心臟監(jiān)護(hù)病房的住院時(shí)間和通氣時(shí)間有關(guān)。值得注意的是，志賀氏埃希菌在區(qū)分 CPB-ALI 患者和非 ALI 患者方面表現(xiàn)最好。結(jié)論我們的研究表明，與術(shù)后非 ALI 患者相比，術(shù)后 ALI 患者在入院時(shí)具有不同的腸道菌群。腸道菌群的菌群失調(diào)可能通過(guò)代謝途徑、群體感應(yīng)和血清中表達(dá)的炎癥因子水平影響 ALI 的進(jìn)展。志賀氏菌 是 CHD 嬰兒術(shù)后發(fā)生 ALI 的潛在預(yù)測(cè)因素。急性肺損傷、先天性心臟病、體外循環(huán)手術(shù)、腸道菌群、生物標(biāo)志物
