雜志名稱：International Journal of Biological Sciences
影響因子：8.2
文章題目：microRNA-130b-3p Attenuates Septic Cardiomyopathy by Regulating the AMPK/mTOR Signaling Pathways and Directly Targeting ACSL4 against Ferroptosis
DOI: 10.7150/ijbs.82287
第一作者：Zhen Qi , Ruhui Liu , Haining Ju , Mengxi Huang , Zhe Li , Wei Li , Yongyi Wang
作者單位：
上海交通大學(xué)醫(yī)學(xué)院附屬仁濟(jì)醫(yī)院
四川大學(xué)華西第二大學(xué)醫(yī)院
上海中醫(yī)藥大學(xué)第七人民醫(yī)院
南京鼓樓醫(yī)院
引用YOBIBIO產(chǎn)品：
U96-5422E Mouse cTnI ELISA Kit
U96-5379E Mouse CK-MB ELISA Kit
文章摘要：
Ferroptosis is a newly identified type of programmed cell death that has been shown to contribute to the progression of septic cardiomyopathy. Although the role of miR-130b-3p as an oncogene that accelerates cancer progression by suppressing ferroptosis has been demonstrated, its role in the regulation of ferroptosis and cardiac injury in Lipopolysaccharide (LPS)-induced cardiomyopathy has not been fully clarified. In this study, we demonstrated that miR-130b-3p remarkably improved cardiac function and ameliorated morphological damage to heart tissue in LPS-induced mice. miR-130b-3p also improved cell viability and mitochondrial function and reduced the production of lipid ROS and ferroptosis in LPS-treated H9c2 cells. In addition, miR-130b-3p significantly upregulated GPX4 expression and suppressed ACSL4 activity in LPS-induced mouse heart tissue and H9c2 cells. Mechanistically, we used database analysis to locate miR-130b-3p and confirmed its inhibitory effects on the ferroptosis-related gene ACSL4 and autophagy-related gene PRKAA1 using a dual-luciferase reporter assay. In addition, we found that miR-130b-3p inhibited the activation of autophagy by downregulating the expression of the AMPK/mTOR signaling pathway. Meanwhile, our results show that RAPA (an autophagy activator) reverses the protective effect of miR-130b-3p mimic against LPS-induced ferroptosis, while CQ (an autophagy inhibitor) plays a facilitative role, suggesting that miR-130b-3p plays an important role in the development of ferroptosis by regulating autophagy in vitro. The findings reveal a novel function of miR-130b-3p in attenuating ferroptosis in cardiomyocytes, providing a new therapeutic target for ameliorating septic cardiomyopathy injury.
鐵死亡是一種新發(fā)現(xiàn)的程序性細(xì)胞死亡類型，已被證明有助于膿毒癥心肌病的進(jìn)展。盡管miR-130b-3p作為癌基因通過(guò)抑制鐵死亡加速癌癥進(jìn)展的作用已被證明，但其在脂多糖（LPS）誘導(dǎo)的心肌病中鐵死亡和心臟損傷的調(diào)節(jié)中的作用尚未完全闡明。在這項(xiàng)研究中，我們證明 miR-130b-3p 顯著改善了 LPS 誘導(dǎo)的小鼠的心臟功能并改善了心臟組織的形態(tài)損傷。miR-130b-3p 還可以改善 LPS 處理的 H9c2 細(xì)胞中的細(xì)胞活力和線粒體功能，并減少脂質(zhì) ROS 的產(chǎn)生和鐵死亡。此外，在LPS誘導(dǎo)的小鼠心臟組織和H9c2細(xì)胞中，miR-130b-3p顯著上調(diào)GPX4表達(dá)并抑制ACSL4活性。從機(jī)制上講，我們使用數(shù)據(jù)庫(kù)分析來(lái)定位 miR-130b-3p，并使用雙熒光素酶報(bào)告基因測(cè)定證實(shí)其對(duì)鐵死亡相關(guān)基因 ACSL4 和自噬相關(guān)基因 PRKAA1 的抑制作用。此外，我們發(fā)現(xiàn)miR-130b-3p通過(guò)下調(diào)AMPK/mTOR信號(hào)通路的表達(dá)來(lái)抑制自噬的激活。同時(shí)，我們的結(jié)果表明，RAPA（一種自噬激活劑）逆轉(zhuǎn)了 miR-130b-3p 模擬物對(duì) LPS 誘導(dǎo)的鐵死亡的保護(hù)作用，而 CQ（一種自噬抑制劑）則起促進(jìn)作用，這表明 miR-130b-3p 發(fā)揮著促進(jìn)作用。通過(guò)體外調(diào)節(jié)自噬在鐵死亡的發(fā)展中發(fā)揮重要作用。研究結(jié)果揭示了 miR-130b-3p 在減輕心肌細(xì)胞鐵死亡方面的新功能，為改善膿毒癥心肌病損傷提供了新的治療靶點(diǎn)。
