雜志名稱：Chemistry & Biodiversity
影響因子：2.9
文章題目：Ameliorative Effect of Morinda officinalis oligosaccharides on LPS-induced Acute Lung Injury
DOI：10.1002/cbdv.202400506
第一作者：Cheng Wang, Cheng Qing, Yanrong Wu, Binbin Liu, and zhenguo Zeng
作者單位：
南昌大學江西醫(yī)學院第一附屬醫(yī)院麻醉與疼痛醫(yī)學中心重癥監(jiān)護醫(yī)學系
江西中醫(yī)藥大學附屬醫(yī)院眼科
南昌洪都中醫(yī)醫(yī)院重癥內(nèi)科
江西省衛(wèi)生健康委員會危重癥護理醫(yī)學重點實驗室
南昌大學傳染病診斷重點實驗室
引用YOBIBIO產(chǎn)品：
U96-1494E Mouse IL-1β ELISA Kit
U96-1511E Mouse IL-6 ELISA Kit
U96-3112E Mouse TNF-α ELISA kit
文章摘要：
Acute lung injury (ALI) is a disease characterized by extensive lung damage and rampant inflammation, with a high mortality rate and no effective treatments available. Morinda officinalis oligosaccharides (MOOs), derived from the root of the traditional Chinese medicinal herb Morinda officinalis, known for its immune-boosting properties, presents a novel therapeutic possibility. To date, the impact of MOOs on ALI has not been explored. Our study aimed to investigate the potential protective effects of MOOs against ALI and to uncover the underlying mechanisms through an integrated approach of network pharmacology, molecular docking, and experimental validation. We discovered that MOOs significantly mitigated the pathological damage and decreased the expression of pro-inflammatory cytokines in LPS-induced ALI in mice. Complementary in vitro studies further demonstrated that MOOs effectively attenuated the M1 polarization induced by LPS. Network pharmacology analysis identified HSP90AA1, HSP90AB1, and NF-κB as key overlapping targets within a protein-protein interaction (PPI) network. Furthermore, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses elucidated the biological processes and signaling pathways implicated in MOOs′ therapeutic action on ALI. Subsequently, molecular docking affirmed the binding of MOOs to the active sites of these identified targets. Corroborating these findings, our in vivo and in vitro experiments consistently demonstrated that MOOs significantly inhibited the LPS-induced upregulation of HSP90 and NF-κB. Collectively, these findings suggest that MOOs confer protection against ALI through a multi-target, multi-pathway mechanism, offering a promising new therapeutic strategy to mitigate this severe pulmonary condition.
急性肺損傷(Acute lung injury, ALI)是一種以廣泛的肺損傷和猖獗的炎癥為特征的疾病，死亡率高，無有效治療方法。巴戟天寡糖(MOOs)是從傳統(tǒng)中藥巴戟天(Morinda officinalis)的根中提取的，以其免疫增強特性而聞名，為治療提供了一種新的可能性。迄今為止，moo對ALI的影響尚未得到探討。本研究旨在通過網(wǎng)絡(luò)藥理學、分子對接和實驗驗證的綜合方法，探討MOOs對ALI的潛在保護作用，并揭示其潛在機制。我們發(fā)現(xiàn)MOOs可以顯著減輕lps誘導的ALI小鼠的病理損傷，降低促炎細胞因子的表達。補充的體外研究進一步證明MOOs可以有效地減弱LPS誘導的M1極化。網(wǎng)絡(luò)藥理學分析發(fā)現(xiàn)HSP90AA1、HSP90AB1和NF-κB是蛋白-蛋白相互作用(PPI)網(wǎng)絡(luò)中的關(guān)鍵重疊靶點。此外，基因本體(GO)和京都基因與基因組百科全書(KEGG)分析闡明了MOOs對ALI治療作用的生物學過程和信號通路。隨后，分子對接證實了MOOs與這些鑒定靶點的活性位點的結(jié)合。為了證實這些發(fā)現(xiàn)，我們的體內(nèi)和體外實驗一致證明MOOs顯著抑制lps誘導的HSP90和NF-κB上調(diào)�？偟膩碚f，這些發(fā)現(xiàn)表明moo通過多靶點、多途徑機制對ALI具有保護作用，為減輕這種嚴重肺部疾病提供了一種有希望的新治療策略。
